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Photo of the publication cover. Pulse-field Gel Electrophoresis Molecular Biology - Help June 11, 2015

The separation of enzyme-digested genomic DNA to generate an organism fingerprint, which is compared to local and national databases to identify strain information and identify potential outbreak clusters.



Photo of the publication cover. Sanger Sequencing Molecular Biology - Help June 11, 2015

The identification of bacterial and fungal organisms by ‘reading’ the DNA letter code and searching against a database library.



Photo of the publication cover. Real-Time Polymerase Chain Reaction Molecular Biology - Help June 11, 2015

The targeting of specific DNA sequences of an organism using short primers and a labeled probe to find matching sequences in a sample and track the amplification of those matching sequences during each round of amplification by measuring the fluorescence of the probe.



Photo of the publication cover. Matrix-Assisted Laser Desorption Ionization - Time of Flight Molecular Biology - Help June 11, 2015

Pure, isolated organisms are placed on a specialized plate and vaporized with a laser. The laser causes proteins to disperse and pass through a vacuum tube to a detector within the instrument.



Photo of the publication cover. Pyrosequencing Molecular Biology - Help June 11, 2015

A short, single strand of DNA is sequenced as its complementary strand is synthesized. The release of pyrophosphates, which occurs during the addition of each base, begins a 3-step reaction which causes visible light to be released.



Photo of the publication cover. Instructions for Bordetella pertussis Samples Molecular Biology - Help February 22, 2012

These instructions explain how to collect a specimen of bordetella pertussis.