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Healthcare-associated infections are recognized as a complication of modern medicine and a source of significant morbidity and mortality among hospitalized individuals. Among the HAIs, Clostridium difficile infection incidence has increased markedly in the past decade and now has become the most common HAI pathogen. Furthermore, Clostridium difficile contributes to health care costs with a recent source estimated the attributable cost to be $3427 to $9960 per episode for acute care hospitals.